The research in my laboratory is focused on understanding the functions and mechanisms of enzymes involved in genome replication and transcription. We use among various methods transient state kinetics (rapid chemical quench-flow and stopped-flow) and fluorescence including time-resolved FRET to study enzymatic reaction dynamics. We combine enzymological studies with structure-function and single molecule studies in collaboration to achieve a detailed understanding of the workings of an enzyme. We use powerful computational methods to model the experimental data to understand and decipher the detailed pathways of enzymatic reactions. We are currently investigating a) replicative enzymes: helicases and DNA polymerase b) transcriptional enzymes: DNA-dependent RNA polymerase. and c) viral replicases: HCV RNA helicase and RNA polymerase.
- Title
- Professor
- patelss@rutgers.edu